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The resulting samples were fractionated on one-dimensional Blue Native gels as described by Giegé et al. [26].
After delipidation, the lipid-free proteins (apoproteins) were fractionated on Sephadex G-150 and DEAE-cellulose.
PCR products were fractionated on 0.8% agarose gels (w/v) and stained with ethidium bromide.
To identify the low-abundance proteins in root tissues, samples were fractionated on 15% PEG and separated by 2D-PAGE.
After detergent treatment, erythrocyte membranes were fractionated on sucrose gradients.
Ribosomes, preribosomes and polyribosomes were fractionated on sucrose gradients as described in [13].
Reactions were fractionated on a 2% agarose gel, stained with ethidium bromide and visualized.
Translation reactions were fractionated on 7.5% or 16.5% SDS-PAGE (Biorad) as indicated.
Samples were fractionated on 11.5% SDS polyacrylamide gels and transfered to polyvinylidene fluoride (PVDF) membranes.
PCR products were fractionated on an agarose gel and visualized with ethidium bromide.
The amplified PCR products were fractionated on a 1% agarose gel.
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