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At 24 h after LPS, lungs were extracted from mice, and nuclear proteins were fractionated for western blot analysis of nuclear Nrf2, indicative of activated Nrf2 [ 11].
At this time blood samples were taken for a head-space analysis (whole blood); additional serum and hemoglobin were fractionated (for details see below).
Cells were fractionated for neutrophils and monocytes using a XE-2100 automated hematology analyzer (Sysmex, Kobe, Japan) in the central laboratory of the hospital.
Extracts were fractionated for 3 h 30 min at 35,000 rpm at 4°C in a Beckman SW41 rotor, and the gradients were recovered in 3 fractions [monosome, light polysome (2 to 5 ribosomes) and heavy polysome (> 5 ribosomes)] using a Brandel gradient fractionator equipped with an ISCO UA-6 flow cell set to 254 nm.
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In one approach, cells are ground up and the substituent chemicals are fractionated for further analysis.
The parent botanical was fractionated for evaluation of potential active components.
We determine how, for a fixed total electron-budget, the available dose should be fractionated for maximum strain mapping precision.
Similarly, differentiated cells can be fractionated for further investigation based upon the lineage restricted expression of specific miRNAs.
Upon reaching 80% confluency, cells were starved overnight and treated the next day with 50 ng/ml FGF19 and 1 µg/ml heparin for 10 min prior to the addition of 10 ng/ml TNFα for 30 min. Cell lysates were fractionated as for EMSA.
Samples were fractionated and stored for further ELISA analysis at 4°C.
To explore further the roles of DNA-PKcs and PP6 in mitosis, HeLa cells were synchronized by thymidine/nocodazole block and extracts were fractionated to enrich for mitotic spindles.
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