Sentence examples for were focused using from inspiring English sources
On both instruments the samples were focused using 20× and 50× objectives.
Incident X-rays ((lambda =) 0.9744 Å, 12,735 eV) were focused using a bent cube root I-beam Si (311) monochromator.
Protein concentration was determined using a PlusOne™ 2-D Quant Kit (Amersham Biosciences-GE) and equal amounts of protein were applied (150 µg) to Immobiline DryStrips (18 cm pH 3 10 linear) and were focused using 50 µA per strip at 20°C with the following conditions: 500 V for 1 m, 4 000 V for 1.5 h, 8 000 V for 25 000 Vh (IPGphor Isoelectric Focusing, Amersham Biosciences-GE).
Proteins were focused using a Protean IEF Cell (Bio-Rad) at 12°C as described [ 83].
The strips were allowed to rehydrate overnight and were focused using a gradient voltage protocol (175 V for 15 min, 175 2000 V ramp for 45 min, and 2000 V for 30 min).
Fifty μg of protein samples (or 300 μg for preparative 2-DE protocol) were loaded in anodic cup and proteins were focused using Ettan IPGphor 3 (pI 3 11 program).
A linearly polarized irradiation laser beam of 1,030-nm 1,030-nmth wavelengthd using a concave lens of 12.5-mm focal length.
The excitation line was focused using a 63x (NA = 0.9) water immersion objective for an integration time of 15 s using an inverted microscope system.
The ejected electrons are focused using a unipolar electrostatic lens and conical electrostatic mirror to form a magnified image on a microchannel plate (MCP).
Excitation light was focused using a 60× water-immersion objective (CFI Fluor; 1.0 numerical aperture; Nikon).
The beam was focused using a 63× objective (Nikon, Japan).
