Sentence examples for were flat mounted to from inspiring English sources

Retinas were flat mounted to orient the photoreceptor cell side upwards in the perfusion chamber (perfused 2 4 ml/min with oxygenated Ames media at 36 ºC).

In this model retinal vaso-obliteration occurs in the central retina when pups are exposed to high oxygen (from P7 to P12) and when returned to room air the relative hypoxia induces neovascularisation with a peak reached at P17. Retinas were flat mounted and stained with isolectin to visualise vessels with neovascularisation occurring at the edge of the central ischaemic area (see Figure 6A).

Cortical slices were flat mounted between glass slides separated by 1- to 1.5-mm spacers and post fixed in 4% paraformaldehyde (PFA) for about 6 h.

Hyperoxia exposed retinas were flat mounted and photographed, then the size of the avascular areas was determined.

After washing in wash buffer, diaphragms were flat mounted on slides with SlowFade Gold antifade reagent (S36936; Invitrogen).

Seven days later, mice were sacrificed, eyes were removed and corneas were flat mounted (n = 5, one flat mount per mice).

After washing in PBS, the retinas were flat mounted in 50% glycerol and photos were taken with a confocal microscope (Leica TCS SP2 Confocal Microscope, Leica, Wetzlar, Germany).

Photoreceptors of the total neural retinas were kept facing up on the slide and the retinas were flat mounted in gel medium for microscopic analysis (Biomeda, Foster City, CA).

Spinal cords were flat mounted in 1 volume glycerol/1 volume 4% PFA for imaging.

Cochleae were flat mounted in Vectashield and confocal images were obtained with a FluoView FV1000 confocal microscope (Olympus).

Retinas were flat mounted with the ganglion cell layer facing upward on Millicell-CM chamber (0.4  μm culture plate insert, 30mm diameter; Millipore,Bedford, MA, USA).