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For these stainings, preparations were fixed for 15 min. in Bouin's fixative (Sigma, L'isle d'Abeau, France).
Dissected tumors were fixed for 2 h in 4% PFA.
After a pause, the audio troubles were fixed for the rest of the event.
Slides of hMSGs, cells, and liver tissues were fixed for 15 min in precooled 4% paraformaldehyde.
The selected parameters were fixed for the 10 min of training.
At day 4 of culture, Fb were fixed for 20 minutes in 1% PFA diluted in Phosphate-buffered saline (PBS).
After 7 days mammospheres were fixed for 20 min at room temperature with 4% paraformaldehyde (Sigma-Aldrich #158127).
Primary root apices of 6-day old seedlings were fixed for electron microscopy after landing.
The mice spleens were fixed for immunohistochemistry 12 h after the challenging irradiation.
Cells were fixed for cell senescence-associated β-galactosidase assay.
These parameters were fixed for all runs.
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