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Numerous bugs were fixed, as well as the inclusion of new archery/spellcasting killcams.
For immunocytochemistry, cells were fixed as above.
Animals were killed in adulthood (60 to 90 days of age), and their brains were fixed as above.
AlexaFluor 594 goat anti-mouse and AlexaFluor 647 goat anti-rabbit IgG were used to fluorescently label RAD51 and CyclinA respectively and the cells were fixed as described.
Cochleae were fixed as described above, post-fixed in 4% paraformaldehyde in 0.1 M phosphate buffer for 2 hr, and decalcified in 5% EDTA.
For immunolabeling of the leaf tissue, samples were fixed as described above, but the secondary fixative step was omitted.
The entrance and exit slit widths were fixed as same for all the measurements.
The refrigerant and the mass flow rate were fixed as R-123 and 0.72 g/s, respectively.
The flow rates of MMS, H2, and CO2 were fixed as 1.7, 47.5, and 0.4 sccm, respectively.
The packet lengths were fixed as follows: DSC 9 bytes, DLY 12 bytes, 12 bytes, and 12 bytes.
To cover all the wetland and feeder areas, 13 sites of Harike wetland were fixed as shown in Fig. 1.
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