Sentence examples for were first trypsinized from inspiring English sources

Adherent cells in exponential phase of growth were first trypsinized (we verified that CEA antigen is not sensitive to trypsin treatment), then collected, carefully washed in PBS 1% BSA and pelletted.

These cells were first trypsinized.

For the T-47D and LNCaP cells, the cells were first trypsinized using 0.25%% trypsin/0.53 mM EDTA (MP Biomedicals, LLC; Santa Ana, CA), re-suspended in fresh media, and then seeded at a concentration of 2.5 × 105 cells/well in 24-well plates.

Adherent cells were first trypsinized and then collected by centrifugation at 700 × g for 5 min at 4°C.

For flow cytometry, cells were first trypsinized, resuspended in PBS with 2% FBS, and transferred to round-bottom 96-well plates for use with the Hypercyt reader.

For the apoptosis of sphere cells, the sphere cells were first formed in the SFM, then trypsinized, and seeded in the suspension dish.

Eluted fractions from the anti-GFP immunoprecipitation (IP) were first precipitated by trichloroacetic acid, then reduced, alkylated, and trypsinized (Promega) (Tian et al. 2007).

The fluorescence of cells were first observed and photographed under an inverted fluorescence microscope, then trypsinized, and collected for flow cytometry analysis.

Cells that had previously been transfected were trypsinized and counted.

First, cells were trypsinized and cell number was determined using a hemocytometer.

Third, NOS-1 cells were trypsinized (trypsin-EDTA, Gibco Lab) and resuspended with α-MEM.