Sentence examples for were first inserted from inspiring English sources
Multi-gene sequences were first inserted into the pcDNA vector (Invitrogen, Carlsbad, CA, USA).
The amplicons were first inserted into the EcoRV site of a pBlueScript (pBK SK-) vector by TA cloning.
The full-length coding sequences were first inserted between the BglII and NotI sites of plasmid pHGB [ 56], and then transferred into plasmid pCHD-1/2 [ 56] by a clonase reaction (Gateway system, Invitrogen) according to the manufacturer's instructions.
The resulting PCR products were first inserted into the vector pPIC9, respectively, and then each SacI/ SalI fragment was excised from the recombinant pPIC9 plasmids and inserted into the Pichia expression vector, pPIC9K.
The cDNAs were first inserted in the pCR2.1 vector (Invitrogen) or pGEM-T Easy vector (Promega), then sub-cloned into BamHI/NotI sites of the pcDNA 3.1 expression vector (Invitrogen).
The religious accommodation provision was first inserted into the bill last year by Representative Todd Akin, the Missouri Republican whose comments on rape helped sink his Senate campaign last fall.
The provision was first inserted in the articles of incorporation of Wynn's predecessor private company which was first started by Wynn and Mr. Okada to build Wynn's Las Vegas casino.
Among common DRM tactics is the installation of hidden or secret files, such as rootkits, on users' computers when a compact disc (CD) or digital videodisc (DVD) is first inserted into their machines.
During IMD processing, the film having the same shape as mold cavity is first inserted into the mold then molten polymer is injected into the cavity.
A new polylinker was first inserted between the BamH1 and EcoR1 sites of pSP72-1.27, giving rise to pSP72BSSPE (Fig. 1A, material and methods).
To insert ALP1 into chromosome II, the ALP1 ORF was first inserted into pCR4 (Invitrogen) and a kanMX6 cassette was inserted downstream of ALP1 into the SpeI site of pCR4, yielding plasmid pKHS332.
