Sentence examples for were extracted upon from inspiring English sources

Briefly, intact cells were harvested 24 h p.t. using 0.02% EDTA and washed twice with PBS, and nuclei were extracted upon incubation in nuclei isolation buffer {320 mM sucrose, 0.1% Triton X-100 and Complete™ EDTA-free protease inhibitor cocktail tablets (Roche) in IB [intracellular buffer: 110 mM KCl, 5 mM NaHCO3, 5 mM MgCl2, 1 mM EGTA, 0.1 mM CaCl2 and 20 mM Hepes (pH 7.4)]} for 5 min on ice.

Several damage indicator variables are extracted upon the simulation of the compressed data stored in memory chips of self-powered sensors.

Water samples were extracted immediately upon arrival using the XAD resin process of Richardson et al. (2008) [for further details, see Supplemental Material (doi 10.1289/ehp.1001965)].

The auditory stimuli were extracted from or modified based upon an existing database of category specific sounds (Engel et al., 2009).

All OGs that contained at least one sequence of a representative of each group were extracted with Perl scripts (available upon request) and concatenated into seven supermatrices that constitute the seven decisive datasets.

Data were extracted from the selected evidence based upon these building blocks.

Upon thawing, cells were extracted with 50 μl of 1 M H2SO4, poly P was purified on PCR purification columns and enzymaticaly digested with ScPpx1, and the released phosphate was colorimetrically quantified exactly as described before [ 42].

Thus, to further confirm the identity of the detected S100A4 variants, regions of interest were excised from gels and in-gel tryptic digestion performed, upon which peptides were extracted and run on MALDI-TOF.

The top and bottom 5% blocks based upon π value were extracted, and the genes in these blocks defined as high- and low-divergence genes, respectively (Additional file 1: Data S7 and S8).

In addition other factors were extracted in order to assess their influence upon graft failure.

Data were extracted using a structured data collection form we designed (this is available upon request).