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Before imaging, samples were extensively washed in PBS + 0.2% Tween-20.
Fixing and permeabilising was performed at room temperature and oocytes were extensively washed with PBS between stages.
After 2 h incubation with exosomes, coverslips were extensively washed by gentle swing 15 times in ice-cold 1× PBS (200 ml), followed by fixing and immunocytochemistry.
Samples were extensively washed in milli-Q water to remove any adsorbed acid.
After removal of the excess solution, the sections were extensively washed with distilled water and then dried in air.
Supernatants were incubated with FLAG-M2 monoclonal antibody-agarose for 1 h and unbound proteins were extensively washed away.
Cells were extensively washed to remove residual virus.
After 2 h, cells were extensively washed, trypsinized and lysed.
Cells were extensively washed and the medium was changed to one containing antibiotics.
Beads were extensively washed and incubated with mouse skeletal muscle homogenate.
The GST-hERK1 or GST-null protein complexes were extensively washed and resolved on SDS-PAGE.
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