Using this approach, Prox1 expression was extensively removed from the developing cochlea (Fig. 5A').
This difference denoted that the lignin locating on the surface of pretreated plant cell walls was extensively removed during the delignification process, therefore the crystalline cellulose underneath was exposed and recognized by the CBM3a-modified AFM tip [ 28].
In recent months, Chinese microblogs such as Sina Weibo have been extensively removing such content from their platforms, stressing that such material is not in keeping with a healthy online culture.
Both untreated and IL pretreated Douglas-fir woodchips showed large cross peaks that can readily be assigned to lignin methoxyl groups δ (H/C) 3.73/55.4, and cellulose (δ 3.49-3.79/60.2, δ 3.37/74.3, δ 3.07/72.6) indicating that these polymers were not extensively removed during pretreatment.
Cells were extensively washed to remove residual virus.
After 3 h, the cells were extensively washed to remove extracellular bacteria and were cultured for 6 days in DMEM.
After 6 8 d of culture, iDCs were extensively washed to remove PIs, and terminally differentiated by incubation with LPS at 1 µg/ml (Sigma-Aldrich, St . Louis MO, USA) for 24 h.
Between each experiment, gold crystals were extensively cleaned to remove LP and PEG for subsequent experiment.
Precipitations were extensively washed to remove unbound proteins and resolved by SDS-PAGE followed by immunoblotting with anti-PARP-1 (Cell Signaling), or anti-GST (Santa Cruz) antibodies.
Following the Endonuclease V digestion, the beads were extensively washed to remove all Endonuclease V. Enzyme carry forward could cause phasing problems, therefore, a guanidine wash was also performed to inactivate residual enzyme.
