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After completion of the extraction, the methanol solvents were evaporated using rotatory evaporator.
Then extracts were evaporated using rotary evaporator under 37 °C and kept in moisture free place until further use.
The purified extracts were evaporated using a rotary evaporator set at 35°C to a volume of 3.5 ml.
Finally, the combined chloroform layers were evaporated using a rotary evaporator under reduced pressure to yield the chloroform extract of C. taii (CFCT, 210 g).
The combined organic solvents were evaporated using a N2 evaporator at 40°C and dried further in a vacuum desiccator over P2O5-KOH for at least 30 min. Finally, the dried residue was derivatized with MSTFA/NH4I/DTE (40 μL; 500 4 2, v/w/w) at 60°C for 20 min, and 2 μL of the resulting mixture was subjected to GC-MS in selected-ion monitoring (SIM) mode.
Solvents were evaporated using a high-vacuum rotary evaporator.
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Solvent was evaporated using rotary evaporator to obtain soluble extracts (Ballantine et al. 1987).
After that, the solution was filtered using filter paper to remove by-products and the solvent was evaporated using rotary evaporator following precipitation into methanol.
The lipid fractions were separated in a clean pre-weighed vial (first wt) and the solvent was evaporated using rotary evaporator.
For those samples extracted with methanol, total supernatant was evaporated using rotary evaporator (Rotavapor RII, BUCHI, Switzerland) and re-dissolved again with 20% methanol.
The supernatant was removed using 0.45 μm syringe filter and the solvent was evaporated using rotary evaporator.
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