In a typical digestive ripening, a batch of as-prepared Au NPs solution was evaporated for about 35 °C by rotary evaporation to get rid of the solvent.
The extract with the solvent was decanted from the soxhlet apparatus and the filtrate was evaporated for the total elimination of solvent using a Rota flash vacuum evaporator at 50°C.
The solvent was evaporated for 20 s using compressed air and a preliminary reading for the unpolymerized material (monomer) was taken using 24 co-added scans and 4 cm-1 resolution.
A two-step, etch-and-rinse adhesive Single Bond 2 (3 M ESPE, St . Paul MN, EUA) was applied, the solvent was evaporated for 20 s, and photoactivation was performed for 20 s using a light-emitting diode unit (Radii Cal SDII, Victoria, Australia) with 600-mW/cm2 600-mW/cm2.
For stimulus, a 10 µl aliquot of a test compound in the desired dose was loaded on a filter paper strip, the solvent was evaporated for 30 s, and the strip was placed in a disposable plastic syringe from which air was delivered to the preparations.
The 133 g of fermented material was divided into three 30-g samples and a 43-g sample, and each sample was evaporated for 30 minutes.
The petroleum ether was evaporated for 10 min, and 1 mL of 20 mM Tris HCl pH 8.3, 1.5 mM KCl, 10 mM DTT, 1 mM PMSF and 0.1% V/V SDS was added.
Though blue-collar, heavy industry jobs that enabled many men to support a family have been evaporating for three decades, the decline from 1989 to 1999 spread beyond jobs in factories, mills and mines to those in offices, stores, warehouses and trucking.
After the addition of CH3CN (2 mL), solvents were evaporated at 55 °C for 1 min at 0.1 bar followed by an evaporation step at 95 °C for 3 min under high vacuum (0.01 bar).
