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The pregnant ewes carrying the GA control lambs were euthanized using an injection of T61®.
Ten fish were euthanized using 0.1% ethyl-m-aminobenzoate methane sulfonate salt (MS-222, MP Biomedicals, Illkirch, France, pH adjusted to 7) followed by decapitation.
Sampled fish were not returned to the experimental population and were euthanized using an over dose (300 ppm) of MS 222.
Animals were euthanized using CO2.
Mice were euthanized using CO2 inhalation.
Animals were euthanized using CO2 inhalation and cervical dislocation.
On day 8 after infection, all mice were euthanized using CO2 asphyxia.
The moribund mice were scored as "dead", and were euthanized using carbon dioxide.
To prepare brain lysates mice were euthanized using carbon dioxide followed by perfusion with PBS.
At 24 hours post-infection, mice were euthanized using forced inhaled CO2.
At the end of the study, the rats were euthanized using isoflurane anaesthesia followed by decapitation.
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