Cells were isolated from collected mammary glands, and mammosphere cultures were established as described previously (see also Supplemental Materials).
The 13C and 15N resonance assignments were established as described in our previous studies32, 36, using a set of 2D and 3D chemical shift correlation spectra including 15N 13Cα, 15N 13Cα 13CX, 15N 13C΄ 13CX, and 13C΄ 15N 13Cα.
Pirh2-overexpressing myeloma cell lines ARP-1-Pirh2, ARK-Pirh2, LP-1-Pirh2 and their corresponding controls were established as described earlier.
Growth curves were established as described previously [41].
Myoblast cultures were established as described previously [12], [39], [40].
Multipotent adult progenitor cells (MAPC) were established as described [30].
Somatic callus lines were established as described by [54].
PCR mixtures were established as described in [13].
Co-cultures of OPCs and DRG were established, as described above.
Stable cell lines were established as described in Supporting Information S1.
ES cell lines were established as described by Matsukage et al. [4].
