Until 2009, the search and identification of inks were essentially performed manually.
Relevant steps of the surgical technique were essentially performed according to the clinical recommendations of osteotomy experts (Lobenhoffer & Agneskirchner 2003b).
Electromobility shift assays were essentially performed as previously described [26].
All histological procedures were essentially performed as described previously [17].
Coprecipitations were essentially performed as described [18], [22], [27], [43].
In vivo ubiquitination assays were essentially performed as described [8].
Immunoblotting procedures and immunoprecipitation experiments were essentially performed as described [33], [42].
cDNA synthesis and qRT-PCR were essentially performed as described [39].
SDS-Page and Western blotting procedure were essentially performed as described previously [1].
Sedimentation assays, light scattering, GTP hydrolysis assays and electron microscopy were essentially performed as described [21].
Generation of genomic representations, library construction, target preparation and image analysis were essentially performed as described previously [17], [18], with the modifications described by Wittenberg et al. [16].
