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Cultivable fungi were enumerated using plate counts.
Cells were enumerated using a Leica fluorescent microscope (DM4000B, Germany).
Colonies were enumerated using OpenCFU software (Geissmann 2013).
Viable cells were enumerated using stuart colony scientific counter (UK).
Microbial cells in the formaldehyde-preserved samples were enumerated using direct microscopic counts.
Comorbid conditions were enumerated using the Elixhauser Comorbidity Score (Elixhauser et al. [1998]).
The cells were enumerated using an epifluorescence microscope (Nikon 80I) at 1000×.
Lymphocytes and their subtypes were enumerated using direct immunofluorescence assays and 4-colour flow cytometry.
These samples were washed in a surfactant solution and the oocysts were enumerated using immunomagnetic separation techniques.
The effluent was collected and the surviving bacteria cells were enumerated using a modified 'Most Probable Number' method.
Cells were enumerated using an epifluorescent microscope and digital camera to determine the number of cells captured on the surfaces.
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