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Patients undergoing total hip replacement were consented, and bone marrow and peripheral progenitor cells were enumerated based on aldehyde dehydrogenase activity and CD34 and CD133 expression.
Bacteria were enumerated based on spectrometric reading at 600 nm, then the inoculum was adjusted to the desired bacterial concentration in PBS.
Endothelial cells were distinguished based on their fluorescent label and cells were enumerated based on a ratio of endothelial cells to CountBright beads per sample.
To examine cells in varying physiological states, bacteria were enumerated based on the Live/Dead BacLight kit, DAPI (4′,6-diamidino-2-phenylindole) staining, fluorescent in situ hybridization (FISH), and colony forming units (CFU).
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Intestinal bacterial density was enumerated based on total colony forming units (cfu) per individual gut.
Introns were sequentially enumerated based on gene orientation.
A 'hidden population' refers 'to a subset of the general population whose membership is not readily distinguished or enumerated based on existing knowledge and/or sampling capabilities' [ 15].
Twenty-four hours later, dead cells were enumerated by flow cytometry based on GFP-positive cells that were 7-AAD-positive.
All residents aged 15 to 75 years from each included household were enumerated, and one of them was chosen based on Kish's method [ 28].
In each selected household all residents aged 15 to 75 yeas were enumerated, and one of them was randomly selected based on the Kish's method.
SFCs were enumerated on-site using a hand-held magnifying glass.
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