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All strains were enumerated after 3, 5, 8 and 14 days at 15 °C.
Results: In the experimental HIV-1 infection model, 5579±4190 CD4+ T cells secreting HIV-1 antigen were enumerated after polyclonal activation.
Then, the LAB3 cells population decreased: 107 and 106 CFU mL−1 were enumerated after 12 days in alginate-caseinate matrix and in alginate matrix, respectively.
Colonies were enumerated after 21-days incubation at 37°C.
Bacteria were enumerated after plating a dilution series onto LB agar.
Y. pestis colonies were enumerated after 48 h of incubation at 28°C.
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When plating mixed cultures, selective BHI agar plates with Listeria mono Selective Supplement I (Sigma-aldrich, Diegem, Belgium) were used for selectively culturing Streptococci at 37°C, whilst K. oxytoca was enumerated after incubating standard BHI plates at room temperature, as Streptococci were not able to grow at room temperature.
Cells from a sector were harvested and enumerated by CFU assay after 24 h, and a second batch of inoculated plates was enumerated after 48 h (Figure 6C).
Intracellular EHEC was enumerated after lysis with 0.1% triton X-100 and plating on TSA.
The number of acini compared to a nontargeting siRNA control was enumerated after 8 days in culture.
Time-kill assay indicated that S. maltophilia ATCC 13637 cells grown in MHB maintained their viability for at least 24 h of incubation, whereas S. maltophilia treated with chlorogenic acid at increasing concentrations (8, 16, and 32 μg mL−1) killed more than 90% of the cells and no viable cells could be enumerated after 12 h of incubation.
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