Sentence examples for were engineered as described from inspiring English sources

These UNG−/− cells were engineered as described and lack expression of both nuclear and mitochondrial UNG isoforms.

For FRET assays on ΔC and ΔN′, GFP at the N terminus and BFP at site B2 within AAA2 were engineered as described (Kon et al., 2005).

Hsp27-CAT construct was engineered as described previously.

For Sp1 protein knockdown, pSuper-Sp1 plasmid was engineered as described [ 25], with the Sp1 target sequence: 5′-CCTGCAGCAGAATTGAGTC-3′ for mouse, and 5′-GAATCGCACA GTCTCTGGT-3′ for human.

The mutants L320A/L321A and I323A/L324A were engineered as previously described [19].

Meningococcal derivatives of Nm2C4.3 expressing IPTG-inducible pilC1 and pilC2 genes were engineered as previously described for a pilC1 inducible strain, allowing tight control of PilC expression [15], with the difference that both recombinant PilC variants carried a 6-HIS tag at the amino-terminal end of the mature protein.

The pLP-1 recombinant plasmid containing rl2 (vLP-1) was engineered as previously described.

HBL100 cells expressing Cx43 (HBL100Cx43) or empty vector (HBL100v) were engineered by retroviral infection as described by Qin et al. [ 34].

The human Isl1-cre eGFP lineage-tracing ESC line was engineered and maintained as described previously.

ZFNs (Sangamo) were engineered for expression in P. falciparum as described in the Supporting Information experimental section.

EGFP-expressing P. agglomerans containing microparticles were engineered using a 1, 2 or 3%% alginate as described above.