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The wheat P450 cDNAs were efficiently transcribed, but no protein or activity was found.
As shown in Figures 1A and 1C, all templates were efficiently transcribed (compare lanes 1 and 2).
Some of the alleles were efficiently transcribed but showed only a weak enzymatic activity with the genes from the B-genome contributing the most to Bx biosynthesis.
Transgenes from the reprogramming cassette were efficiently transcribed, and subsequently dedifferentiation occurred in some of these cells as we previously demonstrated in the case of mouse cell reprogramming [ 25].
These results suggested that MMCT mediated by retargeted MV fusogen could introduce HACs into human normal cells in excellent condition without recognizable aberrations and that all of the transgenes harbored in the HAC were efficiently transcribed enough to fulfill their functions.
We found similar axr3-1NT-GUS actinity in wild type and ibr5 with or without auxin treatment, suggesting that the transgenes were efficiently transcribed in response to the heat stimulus, and that the decreased AXR3NT-GUS activity in ibr5 was not caused by reduced transcription following heat shock.
Similar(54)
Although some genes are regulated by Pol II pausing in close proximity to the TSS [ 65], others are reported to be efficiently transcribed into precursor transcripts, while the efficiency of transcript processing is being regulated [ 66, 67].
In agreement, ATX1 regulated stress-response genes that are not involved in the PtdIns5P/MTM1-pathway (i.e. the NCED3 gene, not shown); the developmentally regulated AG and FLC genes (Figure 6a, b) are efficiently transcribed during dehydration stress, illustrating different patterns of ATX1 activity, and perhaps, regulation.
The PTH gene was efficiently transcribed, processed and translated to mature immunoreactive PTH.
This "replicational selection" should guarantee that genes on the leading strand, particularly the essential ones, are efficiently transcribed [ 5- 8].
Retroviral polyadenylation signals are thought to be inherently leaky as they must allow read-through of the 5' LTR so that the genome can be efficiently transcribed [ 21].
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