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Finally the AAO templates were dissolved with the help of NaOH.
Subsequently, they were dissolved with a given volume of diethanolamine (DEA) and 10 mL of distilled water.
The medium was discarded and the formazan blue crystals formed were dissolved with 50 μl of DMSO.
The supernatant was discarded, and the precipitates were dissolved with 0.2 M PBS (pH 6.0) under stirring for 1 h.
The purified quartz samples (approximately 25 g) were dissolved with HF and spiked with Be carrier (approximately 0.3 mg).
In conventional CL detection, the AuNPs were dissolved with the solution of NaCl-HCl-Br2 to form AuCl4 −, which could catalyze luminol to initial CL reaction.
0.4911 g of ferrous ammonium sulfate were dissolved with DI water, 10 mL of sulfuric acid (1 1) and 5 mL of hydrogen peroxide.
Following this, the MTT solution was decanted off and the formazan crystals formed in the viable cells were dissolved with 200 μL DMSO.
Pellets were dried 5 min in a speed-vac and the precipitates were dissolved with 0.08 % sodium hydroxide and 0.4 % sodium carbonate.
They were dissolved with methanol respectively to get ten reference standards stock solutions (1.0 mg mL-1), and were stored at 4°C.
The resulting formazan crystals and remaining neutral red dye were dissolved with isopropanol or 1% glacial acetic acid in 50% ethanol, respectively.
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