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Cells were dissociated for 5 hours by gentle shaking.
Colonies were dissociated for passaging using trypsin with 3 mm glass beads.
EBs were dissociated for flow cytometric analysis or CD34+ cell enrichment by FACS by the addition of 1 mg/mL collagenase B (Roche) for 2 hours at 37°C, following by vigorous pipetting.
One hour before surgery, hNPCctx-GDNF or hNPCctx neurospheres were dissociated for 10 minutes in Accutase (1 millionillion cells) followed by inactivation with an equal volume of 0.2% trypsin inhibitor.
Carcasses were washed twice in PBS, minced finely, pieces were dissociated for 10 min with rotation at 37°C using trypsin/EDTA solution, triturated and filtered through a 70 μm mesh.
Mammary glands were dissected and minced with scissors, then the cells were dissociated for 8 h at 37°C in EpiCult-B with 5% fetal bovine serum, 300 units/ml collagenase, and 100 units/ml hyaluronidase.
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"I was dissociated for many years".
Differentiation of genetically corrected patient-specific iPS cells [45] might ultimately produce NSC grown as neurospheres that could be dissociated for transplantation to generate both neurons and their supportive glial cells.
The tissue was dissociated for thirty minutes in 20 U/mL activated papain (Worthington Biochemical Corporation).
It seems equally possible that SPB-kinetochore connections must be dissociated for normal execution of prophase I events, e.g. telomere-nuclear envelope attachment, chromosome movement, etc. 5.
Using the same 2-step strategy, we find that also for n-amyl acetate (AM) and 3-octanol (3OCT) innate relative preference and learnability are dissociated (for all statistics, see legend of Figure 3).
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