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Tetrads arising from sporulation of YJL1001 were dissected on a MSM system dissection microscope (Singer Instruments, UK) and one of the ura4+ spores collected as labeled YJL1002.
Brains were dissected on ice under a dissection microscope to remove all traces of the optic lobe and then stored at −80°C.
After 48 hr in sporulation medium, tetrads were dissected on synthetic complete medium using a Zeiss dissection microscope.
In some instances, lung metastases of varying size were dissected on ice, weighed, and counted to assess remaining radioactivity.
The mosquitoes were dissected on day 8 to analyze their midgut infectivity.
Liver tissues were dissected on ice and immediately weighted before being snap-frozen in liquid nitrogen.
B. ignitus worker bees were dissected on ice using a stereo microscope (Zeiss, Jena, Germany).
The anopheline salivary glands were dissected on day 20 22 and stained with dihydroethidium.
Briefly, brains of mouse embryos were dissected on embryonic day 16.5 (E16.5) and the hippocampi were isolated.
Tissues (antennae, legs, wings, thorax, abdomen) from adults of both sexes were dissected on ice under a light microscope.
Transgenic mice were decapitated 14 h after the last stress exposure; brains were dissected on an ice-cold plate and immediately frozen in liquid nitrogen.
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