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Each lane was split into 5 pieces that were digested individually with Trypsin-Gold (Promega) as described above.
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For size estimation, each Bartonella plug was digested individually with enzymes NotI and SmaI, in duplicate, for 5 h and following an overnight incubation according to the appropriate temperature.
Total DNA was digested individually with restriction enzymes (BamH1, Sma1) according to the manufacturer's instructions and fractioned by 0.85% agarose gel electrophoresis (AGE) in TAE buffer (Maniatis et al, 1982).
For each experiment, 100 µg of proteins were digested with trypsin and labeled individually with the appropriate iTRAQ tag.
About 10 μg of genomic DNA samples of five accessions were digested with BamHI and EcoRI individually and fractionated on 1% agarose gel.
Linearized tdna mutant plasmids were digested with XhoI and SpeI, individually transformed into a tdnaΔ:: URA3 strain, and 5- fluoroorotic acid (5-FOA) resistant colonies were isolated.
The pellets were digested with NaOH (1 M) for 1.5 h at 37°C, neutralized, and individually assayed in duplicate for protein content by the method of Peterson [ 27].
Sometimes zoochlorellae are digested by the host.
Food is digested outside the mouth (preorally).
Expect more once Republic is digested.
It was digested using EcoRI and HindIII.
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