Nsphs were differentiated for five days in differentiation medium [DMEM/F-12, N2, 1% penicillin/streptomycin and 0.5% FBS (Invitrogen)].
Bone marrow cultures were differentiated for 1 week with additions of fresh differentiation medium at days 3 and 6.
In an initial experiment, H1 HESCs were differentiated for 30 days with RNA samples taken before and after differentiation.
The cells were differentiated for 6 days in order to reach an optimal cell density suitable for imaging.
The cells were differentiated for 4 days in the presence of CeO2 or Sm-CeO2 (50 μg/mL).
Human neural progenitor cells (hNPC) were differentiated for 4 days in the presence of CeO2 or Sm-CeO2 (50 μg/mL).
C17.2 cells were differentiated for 6 days in the presence of CeO2 (25 μg/mL), Sm-CeO2 (25 μg/mL) or NAC (1 mM).
C17.2 cells were differentiated for 1 and 7 days under continuous exposure to CeO2 (25 μg/mL), Sm-CeO2 (25 μg/mL) or NAC (1 mM).
(B) C17.2 cells were differentiated for 1 and 7 days in the presence of 25 μg/mL CeO2 or Sm-CeO2 nanoparticles.
For the present study, C17.2 cells were differentiated for 1 or 7 days in the presence of CeO2 nanoparticles (25 μg/mL), Sm-CeO2 (25 μg/mL) nanoparticles, or NAC (1 mM).
C17.2 cells were differentiated for and 7 days (10 days for GFAP) under continuous exposure to CeO2 (25 μg/mL), Sm-CeO2 (25 μg/mL) or NAC (1 mM).
