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Cells were incubated for 10 min at RT in the dark and levels of apoptosis were determining by counting 5000 cells using the ImagestreamX imaging flow cytometer with a 488-nm laser at a power setting of 40 mW (Amnis Corporation, Seattle, WA, USA).
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(Team finishes were determined by cumulative times).
Concentrations were determined by spectrophotometry.
Cell numbers were determined by coulter counter.
Optical density were determined by a spectrophotometer.
PGE2 levels were determined by ELISA.
Beating percentages were determined by light microscopy.
Virus titres were determined by plaque assays.
Preliminary RBP4 concentrations were determined by ELISA.
All cell concentrations were determined by hematocytometer.
Thiamine derivatives were determined by HPLC.
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