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Borders were determined with a ruler — arbitrarily.
Protein subcellular localisations were determined with PsortB (www.psort.org/psortb/).org/psortb/
Predictive values were determined with the second-trimester assessments.
Wear rates were determined with the Martell hip analysis software.
Sensitivity and specificity were determined with existing data.
DBF and aortic blood flow were determined with fluorescent microspheres.
Ploidy and DNA indices were determined with flow cytometry.
Cell volumes and their transients were determined with image analysis.
Mitochondria number and cross-section areas were determined with the Image J software (NIH).
The protein concentrations of the supernatants were determined with a Bio-Rad Protein kit (Bio-Rad).
Plasma levels of human F.IX transgene were determined with an ELISA assay29,59.
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