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The bending and compression forces for the microneedle array were determined using an Instron microtester 5848 (Instron, US).
The concentration and quality of RNA were determined using an Agilent 2100 Bioanalyzer (Agilent Technologies).
Total N and C were determined using an Elemental Analyzer (Leco Truspec, St. Joseph, MI, USA).
Plasma insulin concentrations were determined using an ovine specific insulin ELISA kit (Mercodia AB, Uppsala, Sweden).
IL-8 levels were determined using an OptEIA™ human IL-8 ELISA kit (Becton-Dickenson).
The effective wedge angles were determined using an analytical formula.
Pigment concentrations were determined using an Agilent 1100 series high-pressure liquid chromatography (HPLC) system (Agilent Technologies)43.
Available NH4 + and total organic C (TOC) were determined using an Elemental Analyzer (Leco Truspec, St. Joseph, MI, USA).
Urinary NM and free NE were determined using an HPLC method with electrochemical detection and on-line purification (Volin, 1994).
The experimental parameters were determined using an orthogonal experimental design approach.
The coil parameters were determined using an RLC bridge for different feed frequencies.
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