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BECs were detected using an absorption-detection system that uses the F = 2 → F′ = 3 transition of rubidium-87 atoms.
The specific proteins were detected using an ECL detection system.
Proteins were detected using an ECL detection kit (Pierce).
Bound antibodies were detected using an ECL detection kit (Amersham Bioscience KK, Tokyo, Japan).
Secondary antibodies conjugated to horseradish peroxidase (GE Healthcare) were detected using an enhanced chemiluminescence detection system (GE Healthcare).
The bands were detected using an enhanced chemiluminescence detection system (Amersham Pharmacia Biotech).
The immunoreactive proteins were detected using an ECL Western blotting detection system (Millipore, MA, USA).
Protein bands were detected using an enhanced chemiluminescence detection system (Amersham, Buckinghamshire, UK).
Blots were detected using an enhanced chemiluminescence (ECL) detection kit (Amersham).
After three washes with TBST, proteins were detected using an enhanced chemiluminescence (ECL) detection kit (Beyotime).
Apoptotic cells were detected using an Annexin V-FITC Apoptosis Detection Kit (BD Biosciences, San Jose, CA, USA).
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