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After treatment, cells were detached with trypsin, collected by centrifugation and subsequently resuspended in PBS.
CHO-K1 cells were detached with 4 mM EDTA at 37 °C and harvested in PBS.
Cells were detached with 0.1% trypsin-EDTA, washed, and triturated to a single cell suspension in 10% FBS in PBS.
Passage 4 MSCs were detached with 0.05% EDTA in 0.1% phosphate buffered saline (PBS) and rinsed with 0.1% PBS.
The monolayer cells were detached with trypsin ethylene diamine tetra acetic acid (EDTA) to make single cell suspensions.
Cells were detached with 0.25% trypsin/EDTA, washed in PBS.
After 3 days, cells were detached with trypsin/EDTA and washed once with PBS.
Briefly, ES cells cultured on fixed NIH-3T3 cells were detached with trypsin 0.5 mM EDTA.
When the cells reached 70 80% confluency, the cells were detached with trypsin-EDTA and washed.
After 24 h, they were detached with trypsin and counted on a Neubauer's chamber.
Bound IE were detached with the pipette stream and returned to culture.
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