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Cells were detached by Macrophage Detachment Solution DXF (PromoCell) following the manufacturer's protocol.
U937 macrophage-like cells were detached by Macrophage Detachment Solution DXF (PromoCell) following manufacturer's protocol.
The adherent primary hepatocytes were detached by scraping and resuspended in phosphate-buffered saline (PBS).
AT-cells were detached by vigorous pipetting in PBS/5% FCS and harvested.
However, C. parvum oocysts captured on the glass slide were detached by repeating washing steps.
During subculture, cells were detached by trypsinization when they reached 80%% confluency and split (1 4).
100 μl/well medium containing HepG2 cells (5 × 103) were cultured, after 48 h cells were detached by trypsin.
The prepared cells were detached by treatment with 0.25% trypsin and passaged into cultural flasks at 1 × 104/cm2.
Cells were detached by incubation with 0.25% trypsin-EDTA solution.
The cells were detached by the addition of versen or trypsin-EDTA, respectively.
The macrophages were detached by gently pipetting the PBS across the dish.
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