The system was designed to copy state-of-the art surgical practice, but in tests involving living pigs, it proved capable of outperforming its teachers.
Even though the Synthon FOGA was designed to copy and compete with Copaxone, the active substances were found to be similar in only 7 of the tested 14 (50%) methods (similar is defined as within approved specifications or within the inherent microheterogeneity range of tested Copaxone batches, or not showing statistically significant differences).
The software was designed to copy over its users' "primary email addresses" - the ones they used to log into the social network.
Two paralog ratio tests (PRTs) (Armour et al. 2007) were designed to measure HPR copy number, by identifying paralogous segments of the haptoglobin region using the BLAST-like Alignment Tool (Fig. 1).
Based on available sequence information and suitability of priming sites, primers for the first round of PCR were designed to amplify both copies of Ara h 2, both copies of Ara h 1, or one specific copy of AhFAD2.
Digital technologies were designed to enable perfect copies; they were not designed to enable control over these copies.
PCR primers were designed to distinguish between copies using Oligo 6.82 (Molecular Biology Insights, Cascade, CO) [see Additional file 6].
Based on the available sequence information in GenBank, primers 1306 and 1307 were designed to amplify both copies of Ara h 1. Indels near the 3' end of the open reading frame allowed us to design gene-specific primers 1308 (Ara h 1.01) and 1309 (Ara h 1.02).
Two primers, OPR1A4-F1 (5'-TCACTACCACACCACTCAG-3') and OPR1A4-R1 (5'-CATCTAATTAGTGTCCTGCA-3') were designed to amplify the forth copy of TaOPR-A1 with 1,717 bp in size (GenBank KF035081).
The final piggyBac vectors constructed for this study were designed to have an additional copy of a highly AT-rich (83% A+T), putative transcriptional activator derived from the piggyBac 3' internal domain [ 4, 18].
