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Most primers were design from EXT repeats characterized in other chromosome extremities.
The primers were design from 16S ribosomal RNA (16S rRNA) gene of C. difficile using Gene Runner software (Version 3.01) and at the NCBI using the experimental GENINFO BLAST Network Service to assess degree of homology between these primers and other reported sequences and at the end were obtained from CinnaGen Co, Iran.
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Machines were designed, and they were designed from without.
Primers were designed from the 3′ region of the gene to ensure gene specificity.
RT-QPCR primers were designed from the respective sequences and confirmed by direct sequencing as described.
Section 'Cloud monitoring systems' surveys monitoring tools which were designed, from the outset for cloud computing.
Fifty-six percent of probes were designed from CDS and 44% were designed from ESTs.
AMPs and pathway gene primers were designed from sequenced cDNA or adopted from [17], [18].
Primers were designed from cloned sequences via Methprimer [77], and were used in nested reactions.
Degenerate primers were designed from an alignment of all vertebrate LDH-b proteins.
Primers were designed from the rpoD sequence of the X. campestris pv.
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