The remaining 12 µL were depleted of rRNA (NEBNext rRNA Depletion Kit) and used for library preparation (NEBNext Ultra RNA Library Prep Kit).
Plasma samples were depleted of albumin using AlbuVoid Albumin Depletion Kit (Biotech Support Group, Monmouth Junction, NJ, USA).
To this end, sensory nerves were depleted of neuropeptides by neonatal treatment of rats with capsaicin.
To ensure that the generation of these CTL responses was indeed dependent upon CD4+ T cell help, mice were depleted of either CD4+ or CD8+ cells before immunization.
Moreover, ECV304 cells that were depleted of Fe with the chelator deferoxamine (DFO) demonstrated increased Pb transport.
In the new treatment, animals whose brains were depleted of dopamine had tiny electrodes, the size of a fingernail, implanted on their spinal cords.
Mice were depleted of B cells by intraperitoneal injection of 250 μg of anti-CD20 antibody (Thermo Fisher Scientific, Waltham, MA).
To this end, mice with gBT-I.Thy1.1 TRM cells were depleted of TCIRC cells and, more than 1 week later, were challenged with B16.gB cells.
Brain membranes were depleted of ankyrin, spectrin, and other peripheral membrane proteins by a brief incubation in 0.1 M sodium hydroxide.
Culture supernatants of IL-1-treated human umbilical vein endothelial cells (HEC) were depleted of retroviral p15E-related Ag with immobilized anti-p15E mAb.
Briefly, splenocytes were depleted of erythrocytes by treatment with ammonium chloride (0.1 M, pH 7.4).
