Sentence examples for were denaturing from inspiring English sources

For single-step RT PCR, reverse transcription (48°C for 50 min) was followed by initial denaturation at 95°C for 2 min. Cycling profiles used were: denaturing at 95°C for 30 s; annealing at 55°C for 1 min and extension at 72°C for 2 min. A total of 30 cycles was used, followed by a final extension step of 5 min at 73°C.

The conditions for PCR were: denaturing at 94°C for 2 min, annealing for 40 s at 57°C (GAPDH) or 60°C (FAAH, CB1) followed by elongation at 72°C for 60 s; in subsequent cycles denaturing was performed at 94°C for 40 s.

The thermal cycles were: denaturing at 94°C for 30 s, annealing at 60°C for 30 s, and extension at 72°C for 90 s.

The instrument settings were denaturing at 95°C for 10 min, 45 × denaturing at 95°C for 30 s, annealing at 59°C for 30 s, and elongation at 72°C for 8 min for (PPAR γ and β-actin).

PCR reaction conditions were: denaturing for 5 min at 94°C, followed by 94°C for 30s, annealing for 30s, and 72°C for 1 min per 1 kb, 35 cycles, with a final 7 min elongation step at 72°C.

The conditions of amplification were denaturing at 95°C-5 min, 35 cycles at 94°C-1 min, 62°C-1 min, 72°C-1 min, and a finally termination at 72°C-10 72°C-10e aminified fragments were separated on 1.5% agarose gel elecThephoresis amplifieded with ethidium bromide.

By doing this they are denaturing heat sensitive vitamins and nutrients in the food... then they add vitamins back in so the nutrition labels will be appealing to consumers.

Thermal cycling was: denaturing at 96°C for 20 seconds, annealing at 55°C for 45 seconds and extension at 72°C for 2 minutes for a total of 35 cycles.

All gels displayed in this article are denaturing gels.

An alternative approach to enrich for CpG-rich DNA is denaturing HPLC (DHPLC) [ 78].

Most effective was denaturing the input DNAs, and snap cooling on ice, prior to inclusion in the PCRs.