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MDM were cultured over an eight-day period with routine changing of culture media every two days.
Bovine knee chondrocytes (BKCs) in genipin-crosslinked CG scaffolds were cultured over 28 days for assessing the constructs.
C2C12 cells were cultured over a period of 72 h to study the effect of topographical cues on cell morphology.
Therefore, to test this material for NP regeneration, bovine NP cells were encapsulated in 1% w/vol HA hydrogels at either a low seeding density (20 × 106 cells ml−1) or a high seeding density (60 × 106 cells ml−1), and constructs were cultured over an 8 week period.
To further examine the growth rate under nitrogen enriched conditions the microalgae were cultured over 12 days and sampled periodically.
In this experiment, rat cortical neurons were cultured over a 60 electrode MEA electrode grid shown earlier in Figure 1.
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For the cell stimulation experiments strain AP 76 and its ApxII-a toxin-deficient mutant were cultured over-night at 37°C in PPLO medium (Difco GmbH, Augsburg, Germany) with supplements as previously described [ 35].
These microdevices have been optimized in order to simultaneously (i) enhance signal-to-noise ratios, as required electrochemical monitoring, by defining appropriate electrodes geometry and size, and (ii) provide surface conditions which allow cells to be cultured over during one or two days, through apposite deposition of a collagen film.
To explore TG2 involvement in angiogenesis further, we next used the well-established co-culture model whereby dermal human fibroblast are cultured over 12 14 days with HUVEC cells resulting in highly branched tubule formation.
The WRC was cultured over 3 years in a paddy field with naturally abundant level of As.
This is apparent in B lymphocyte cell lines which can be cultured over many population doublings.
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