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In this study, 54 Greek faba bean populations were cultivated over a 3-year period.
Original UC-derived primary cultures and subpopulations of CCE-enriched small- and large-sized cells were cultivated over the same period for 6 days post elutriation.
IL12-4 and its parental genotypes were cultivated over three consecutive years (2006-2008) in a greenhouse at the Department of Soil, Plant, Environmental and Animal Production Sciences at the University of Naples (Portici, Italy).
The two pathogenic fungi Penicillium expansum and Colletotrichum acutatum were cultivated over 10 days, and enzyme activities in the culture broth were analysed using four different CPH substrates: (A) yellow CPH-galactomannan, (B) yellow CPH-lichenan, (C) yellow CPH-β-glucan from barley, (D) yellow CPH-arabinoxylan.
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Wells were punched into LB-agar plates and were inoculated with 400 μl of E. coli (XL1-blue) suspension in LB medium and the cells were cultivated over-night at 37°C as a lawn on the plates sLOX-1 (Sigma) was diluted with 50 mM potassium phosphate buffer, pH 7.0, and 25 μl of the lipoxygenase solution was applied into the wells in the agar at final amounts ranging from 8 ng to1 μg.
Less easy to excise is the lingering view of Africa that has been cultivated over the last several decades.
In Italian opera up to Aida (1871), the aria was cultivated over a longer period than in German opera.
Knowledge and experience that transcend international boundaries are something that is cultivated over a lifetime, not something that is attained over a summer or a semester.
Altogether, the considerable differences in style imply that the art was cultivated over a sufficiently long period to have multiple ramifications.
The support that some parents seemed to show for the union appears to have been cultivated over a period of months as the possibility of a strike grew.
These ancient grapes have been cultivated over millennia, but they had largely faded from prominence by the middle of the 20th century.
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