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For RSV, Vero cells were cultivated as 1 × 10 cell/well in 96-well flat bottom culture plates in RPMI-1640 culture medium and for HSV-1, MRC-5 cells were cultivated as (3 × 10 cell/well) in 96-well flat bottom culture plates in RPMI-1640 culture medium.
The cells were cultivated as continuous cultures with a dilution rate of 0.005 day−1.
About the same time, rubber and coconuts also were cultivated as plantation crops.
Cells were cultivated as described above; however, before exposing cells to KE medium pH 5.8+lysine, they were incubated for 5 min with 400 µg/ml chloramphenicol.
Pure cultures were cultivated as controls and the concentration of nitrogen was also varied (NH4Cl: 50, 100, 200 and 375 mg L−1).
HUVECs were cultivated as described before [22] on gelatin (1 % in phosphate buffer saline (PBS)) coated T25 and T75 flasks in M199 (containing 20 % FBS, 0.675 % bovine pituitary extract (BPE), 0.125 % heparin and 1.25 % penicillin/streptomycin).
They were cultivated as described [31].
Nematode strains were cultivated as described previously [27].
For the drug transport studies the pBCEC were cultivated as described before.
Human embryonic kidney cells (HEK293T), human neuroblastoma cells (SH-SY5Y) and HeLa cells were cultivated as described previously [14], [58].
AD49 bacteria were cultivated as described in [12] and bacteria from 400 µl solution were pelleted and resuspended in 50 µl of PBS.
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