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Half of the cells were cryopreserved for 3 months.
Two tissue specimens were cryopreserved for >1 month before successful generation of prostate xenografts.
Cells were cryopreserved for 24 h and cell viability post-freezing and thawing was quantified by trypan blue exclusion assay.
Further, normal keratinocyte (HaCaT) and osteosarcoma (Saos-2) cells seeded on PVA-Car scaffolds were cryopreserved for 15 days and their viability was checked at regular interval of 3 days (0, 3, 6, 9, 12, 15 days) through MTT assay and fluorescence microscopy.
Frozen lymphocytes were cryopreserved for immunologic and genetic testing.
The remaining cells were cryopreserved for activation of EBV specific CTLs once the autologous LCL became available.
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Sperm, which does little at -196C, could be cryopreserved for centuries and still safely thawed to fertilise an egg, say experts.
Objective: To report successful pregnancy from embryos that had been cryopreserved for 8.9 years.
We hypothesized that organoid units (OU), multicellular clusters containing epithelium and mesenchyme, could be cryopreserved for delayed production of TESI.
Cloning systems are characterised by allowing PCR product incorporation (Bernard 1996) into a circular plasmid; this can be further propagated to obtain an appropriate number of DNA copies whose integrity can be verified by sequencing and may be cryopreserved for future use (Gruber et al. 2008).
Therefore, somatic cells of endangered or extremely valuable animals have been cryopreserved for maintenance of genetic resources [6] [8].
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