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Total yeast and mold were counted using the surface plate method and reported as CFU (colony forming units) per milliliter (ISIRI 10899-2) [19].
Cells were counted using the ImageJ software.
Nuclei were counted using the manual count option in Metamorph.
Cell numbers were counted using the MTS assay (Promega).
Capillaries were counted using the software IP lab 3.2.4.
For survival assays, cells were counted using the Countess system (Invitrogen, Carlsbad, CA).
The total nucleated cells of the femur and the tibia were counted using the Hemavet® counter.
Plates were counted using the AID Autoimmun Diagnostica GmbH ELISpot reader (Strassberg, Germany) and software.
The iba-1 positive cells were counted using the count tool.
Callose spots were counted using the ImageJ software (http://rsb.info.nih.gov/ij/) [82].
Within each field the total number of neurons within our two-dimensional plane were counted using the Nissl stained sections.
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