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Peak areas were calculated in El Maven (https://elucidatainc.github.io/ElMaven/) and stable isotopic measurements were corrected against the naturally occurring isotopes for each metabolite measured.
The hypocretin contents were corrected against protein concentrations.
Signal intensities were corrected against the membrane background.
Urinary amino acid concentrations were corrected against creatinine values.
The assay results were corrected against β-gal activity assayed using Promega β-gal reporter kit.
Absorbance levels from drug treated cells and untreated controls were corrected against medium only blank controls.
Similar(43)
For each replicate, the radioactivity of the samples was corrected against a blank which corresponded to the pre-fixed sediment cores submitted to the protocol described above.
Gene expression was corrected against GAPDH and GUS mRNA level in each sample.
The selected genes relative expression was corrected against GAPDH and GUSB genes as endogenous controls.
Hence, it must be corrected against bias coming from multiple hypothesis testing to determine statistical significance.
The EGFR mRNA expression level was corrected against GAPDH mRNA in both cell lines.
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