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The chimera mice were continuously bred in B6 background and the offspring shows typical Mendalian distribution of +/+, +/− and −/− genotypes (data not shown).
Six control female FVB mice were continuously bred separately.
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Worms can be continuously bred in the lab throughout the year using a well-developed culture protocol (Hauenschild and Fischer 1969; a detailed description in English is available at http://www.uni-giessen.de/~gf1307/breeding.htm).htm
The Kyoto-Runx3 KO we sent to Israel in 2010 have been continuously bred for about 10 years, which may have resulted in exon 4 skipping and explain why Levanon et al could not observe LacZ staining in GIT epithelium of Kyoto-Runx3 KO (Levanon et al, 2011).
The fish were bred for at least 6 months, and females and males were continuously maintained in a ratio of 1 2.
The flies were continuously active.
Subjects were continuously monitored by trained staff.
Temperature and ECG were continuously monitored.
were continuously measured.
Samples were continuously collected.
were continuously infused.
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