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Plasmids expressing Polo::GFP, Feo::GFP and Feo::PtA under the control of the Actin5C promoter were constructed used Gateway® technology (Invitrogen) as described [24].
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Phylogenetic trees were constructed using the MEGA5 package69.
The genome maps were constructed using GCView57.
Subsequently, all point histograms were constructed using FitMaster.
All other PMFs were constructed using the transition rates method.
Images were constructed using ImageJ software (NIH, Bethesda, MD, USA).
The haplotypes were constructed using those SNPs.
Standard curves were constructed using dextran m.w.
Index files were constructed using the bismark_genome_preparation command and the entire reference genome.
Tobacco BY-2 cells expressing CaMV 2 × pro35S::19E5scFv-GFP (H3K9ac-mintoboy-GFP) were constructed using the following procedures.
To study mecD expression, two plasmids were constructed using S. aureus-E.
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