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The stained nuclei were washed twice with PBS, and were consequently observed under an IX71SIF-3 fluorescence microscope at 350 nm excitation and 460 nm emissions.
The culture medium containing compounds was removed, and the cells were fixed in 4% paraformaldehyde for 10 min. The cells were washed twice with PBS, and were consequently stained with 0.5 mL of Hoechst 33258 staining for 5 min. The stained nuclei were washed twice with PBS, and were consequently observed under fluorescence microscope at 350 nm excitation and 460 nm emissions.
Microtubules were consequently observed to undergo catastrophe within several seconds upon dilution, much shorter than the time to catastrophe during growth and arguing for a small stabilizing cap whose size does not scale with the growth rate.
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A selectivity loss was consequently observed.
A slightly different clustering of metabolites was consequently observed (Figure 1B and Figures S3, S4).
A 130% increase in the expected mortality was consequently observed.
An increase of SOD (albeit slight) and catalase enzymatic activity, after 24 h, was consequently observed.
Fear of hypoglycaemia is consequently commonly observed insulin users [ 10].
Substantial head motion artifacts were observed in one subject whose data were consequently excluded from the analyses.
Many were consequently struggling financially.
"They were consequently marginalized within the firm".
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