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Peak fractions were concentrated with centrifugal concentrators (Vivaspin, Sartorius) and loaded onto a HiLoad Sephacryl S200 16/60 column (GE Healthcare) equilibrated in 20 mM CHES, 250 mM NaCl, 10% glycerol, 5 mM DTT, pH 9.0.
Prior to transducing cells, virus particles were concentrated with Vivaspin 20 concentrators (Sartorius Stedim Biotech GmbH, Goettingen, Germany) according to the manufacturer's instructions.
Peptide fractions were concentrated with a vacuum concentrator (Eppendorf), and kept in low-binding tubes at -20°C until further analysis.
Pure Irga6 proteins were concentrated with Vivaspin 20 centrifugal concentrators (Sartorius).
Dynabeads M-280 Streptavidin (Dynal) were concentrated with a magnetic particle concentrator (MPC) (Dynal) and washed twice in Buffer T (10 mM Tris (pH 7.5), 1 mM EDTA, 1 M NaCl).
Protein containing fractions were subjected to size exclusion chromatography (Superdex 75; GE Healthcare, Munich, Germany) in B1 buffer (50 mM Tris/HCl pH 7.4, 5 mM MgCl2)/2 mM DTT. Irga6 containing fractions were concentrated with Vivaspin 20 centrifugal concentrator (Sartorius, Goettingen, Germany).
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Before use, LDL was concentrated with Centricon 10 concentrators (Amicon, MA) to a final concentration of about 7 mg/mL protein.
The supernatant was concentrated with a gas blowing concentrator.
Total RNA was concentrated with a DNA100 SpeedVac Concentrator (Savant) and reverse transcribed using the High-Capacity cDNA reverse transcription (RT) kit (Applied Biosystems Inc., Foster City, CA).
Pooled PrimPol was concentrated with the Vivaspin protein concentrator.
The eluted antibody was concentrated with Amicon Ultracel-50 k concentrators (Millipore, Billerica, MA, USA) and dialysed against PBS.
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