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Sequences obtained as described above were complemented using a classification-based search for Hymenogaster ITS sequences in GenBank (http://ncbi.nlm.nih.gov/).nih.gov/
These newly engineered mutant strains, PAO1-fadD1::FRT, PAO1-fadD2::FRT, and PAO1-ΔfadD2D1::FRT, were complemented using the relevant gene(s) on the miniCTX2 single copy integration vector as described previously [56].
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This can be complemented using information obtained by much more accurate sensors such as kinect.d.
The data are complemented using institution-level information on financial and logistical support for entrepreneurial activities.
These basis functions are complemented using a local, parameter dependent, weighted eigenvalue problem.
5448ΔdltA was complemented using plasmid pdltA, as described [13], creating 5448ΔdltA pdltA).
The methods described earlier can be complemented using gene expression data.
JN24.6 was complemented using the same vector by performing cotransformation with the hygromycin resistance gene-containing plasmid pAN7.1.
GC-MS metabolite profiling (primary metabolites) was complemented using HPLC-QTOF-MS analysis of mostly secondary metabolites.
The PPI map was complemented using data obtained from STRING (Franceschini et al, 2013) using experimental data and 0.4 confidence as parameters.
This approach was complemented using the available protostome and Ciona EST data to identify putative N and C terminal ends of the protein (Additional file 1).
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