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To select potential P. fluorescens Pf0-1 clones that were complemented for their growth deficiency, all transconjugants were replica-plated to agarose plates containing 5 mM citrate and 10 mM β-valine as sole carbon and nitrogen sources.
glutamicum shuttle vector pC2 [ 15], and the transformants were checked for temperature-sensitivity and lysozyme-sensitivity. Strains KY9704 and KY9706, which showed both lysozyme-sensitivity and temperature-sensitive growth, were complemented for lysozyme-sensitivity and temperature-sensitivity by pHLS4 like KY9714.
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"I think our guys should be complemented for the way they have caught what could be a potentially serious injury, one in which you would have been looking at two or three months out," Watkinson said.
"We have, time and time again been complemented for the measures we have put in place by RFL representatives, so have taken this complete lack of support with surprise and shock," he added.
The QS mutants could all be complemented for their swarming deficieny; the AHL synthase mutants were chemically complemented by providing the signal molecules in the media whereas the lasR and rhlR mutants were complemented with the cosmids pIB101 and pIB103 [26] respectively (data not shown).
Provision of cCBT may be complemented, for example, by the inclusion of collaborative care protocols to provide scheduled patient follow-ups.
These patients were complemented by data for 212 women who had ultrasound fetal assessments of less than 4,000 g.
The blast searches were complemented by searches for distant homologs similar to the procedure described in [ 63].
These measurements were complemented with pedon measurements for soil texture, bulk density, and other biogeochemical properties at the same locations.
They can be complemented by for example host response, stromal or even NF-кB specific gene expression signatures [ 22- 25].
React is complemented by Diode5 for application state management.
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