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For the competition ELISA 10 nM of 22C4 scFv were competed with increasing amounts of recombinant Aβ1-42.
Positive controls were competed with DNase 1 and negative controls were completed without the terminal deoxynucleotidyl transferase enzyme.
Nine hundred nanograms of each probe were competed with 12-fold excess human Cot-1 DNA (1mg/ml; GibcoBRL).
Complexes formed on radiolabelled DBA/2J or 129B6 probes were competed with increasing fold molar excess of either unlabelled DBA/2J DNA or unlabelled 129B6 DNA.
For the last round, the phages bound on GST-RhoBQ63L beads were competed with soluble wild type recombinant RhoB (80 μg).
Additionally, experiments were performed in which reactions of 14 with TruD and ThcD were competed with substrates 2 (TruLy1), 8 (TruLy2 RSI mutant), and 15 (PagE6), using the same methods that were employed earlier to define RSI.
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