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The dried peptides were cleaved from the biotin portion of the ICAT reagent with the cleaving reagents at 37°C for 2 hours.
PAs were cleaved from resin by shaking in a solution of 95% TFA, 4% TIPS, 1% water.
SCNPs on the colloidal particles were cleaved from the surfaces of the colloidal particles, leaving thiol groups on the surfaces.
For these applications, the proteins were cleaved from GST with thrombin (Sigma) on the beads and thrombin was subsequently removed with benzaminidine-agarose beads (GE healthcare).
The graft chains were cleaved from the glass filter by the HF treatment in the presence of a phase transfer catalyst.
Still, the pullback closes a fraught chapter in the relationship between the two countries, one majority Hindu, the other Muslim, which were cleaved from one another in the partition of 1947.
The substrates were cleaved from epi-ready wafers and all handling and depositions were conducted in a clean room facility.
The DOTA-peptide conjugates were cleaved from the solid support, and the lateral protecting groups were removed by TFA treatment.
Subsequently, the chelator-conjugated peptides were cleaved from the resin (TFA/TIBS/H2O) and simultaneously deprotected to yield 7, 8 and 9.
The respective labeling precursors were cleaved from the resin using TFA, with concomitant removal of acid labile protecting groups (54 63% yield after RP-HPLC purification).
Subsequently, the as-prepared peptides were cleaved from the resin using standard trifluoroacetic acid (TFA) cleavage procedures in TFA with 5% H2O followed by multiple ether extractions.
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